Please visit the above links to have a more detailed look at our research projects. Below is a list of Centers that we are leading or affiliated with.
NSF STC EBICS at MIT/GT/UIUC - Emergent Behavior of Integrated Cellular Systems
NSF CiiT (I/UCRC) at UIUC - Center for Innovative Instrumentation Technology
NSF NSEC at OSU - Center for Affordable Nanoengineering for Polymeric Micro and Nanodevices
Detection of Viable Pathogens Using Label-Free Electrical Detection of Nucleic Acid Amplification
Leveraging developments on electrical label-free detection of amplification and expertise in microfluidic systems, we plan to continue the development of an automated microchip for detection of pathogens. Our group has previously demonstrated specific detection of food borne pathogens in a silicon microarray. A primer dehydration protocol enables detection of multiple genes when the sample is partitioned in micro-reactors with different primer sets. In addition, we have shown that the amplification reaction can be electronically detected using ion-sensitive field effect transistors that monitor the electrolyte acidity. We now look to take advantage of the intrinsic scalability of electronics and the arraying technologies that we have developed to do electrical detection of multiple reactions. This will require creating platforms for parallel measurement of transistors and the development of a robust on-chip reference electrode that overcomes the current requirement of having an off-chip electrode which is limiting the number of parallel reactions that can be performed. Additionally, the incorporation of a new pre-amplification step will discriminate live vs. dead pathogens. We will study on-chip performance of viable qLAMP and the viability of performing the treatment on a microfluidic device. In this way, using electronics and microfluidics, we will develop a DNA amplification system for the detection of foodborne pathogens outside a laboratory setup.